Selected publications

Wang, F., Ding, P., Liang, X., Ding, X., Brandt, C. B., Sjöstedt, E., Zhu, J., Bolund, S., Zhang, L., de Rooij, L., Luo, L., Wei, Y., Zhao, W., Lv, Z., Haskó, J., Li, R., Qin, Q., Jia, Y., Wu, W., Yuan, Y., … Luo, Y. (2022). Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level. Nature communications13 (1), 3620. doi.org/10.1038/s41467-022-31388-z

Noer JB, Hørsdal OK, Xiang X, Luo Y, Regenberg B. Extrachromosomal circular DNA in cancer: history, current knowledge, and methods. Trends Genet. 2022 Mar 8:S0168-9525(22)00034-8. doi: 10.1016/j.tig.2022.02.007. Epub ahead of print. PMID: 35277298. (This review provides a comprehensive summary of the eccDNA and its roles in cancers.)

Corsi GI, Qu K, Alkan F, Pan X, Luo Y, Gorodkin J. CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context. Nat Commun. 2022 May 30;13(1):3006. doi: 10.1038/s41467-022-30515-0. PMID: 35637227; PMCID: PMC9151727.

Karlsson M, Sjöstedt E, Oksvold P, Sivertsson Å, Huang J, Álvez MB, Arif M, Li X, Lin L, Yu J, Ma T, Xu F, Han P, Jiang H, Mardinoglu A, Zhang C, von Feilitzen K, Xu X, Wang J, Yang H, Bolund L, Zhong W, Fagerberg L, Lindskog C, Pontén F, Mulder J, Luo Y, Uhlen M. Genome-wide annotation of protein-coding genes in pig. BMC Biol. 2022 Jan 25;20(1):25. doi: 10.1186/s12915-022-01229-y. PMID: 35073880; PMCID: PMC8788080. (The first body-wide classification of protein coding gene expression in pig tissues and organs)

Xiang X, Corsi GI, Anthon C, Qu K, Pan X, Liang X, Han P, Dong Z, Liu L, Zhong J, Ma T, Wang J, Zhang X, Jiang H, Xu F, Liu X, Xu X, Wang J, Yang H, Bolund L, Church GM, Lin L, Gorodkin J, Luo Y. Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning. Nat Commun. 2021 May 28;12(1):3238. doi: 10.1038/s41467-021-23576-0. PMID: 34050182; PMCID: PMC8163799. (This study reports the CRISPRTRAP-seq method for high throughput quantification of CRISPR gRNA activity in cells. The study also reported the generation of new deep learning based tool, CRISPRon, for prediction of CRISPR on-target targeting activity.)

Karlsson M, Zhang C, Méar L, Zhong W, Digre A, Katona B, Sjöstedt E, Butler L, Odeberg J, Dusart P, Edfors F, Oksvold P, von Feilitzen K, Zwahlen M, Arif M, Altay O, Li X, Ozcan M, Mardinoglu A, Fagerberg L, Mulder J, Luo Y, Ponten F, Uhlén M, Lindskog C. A single-cell type transcriptomics map of human tissues. Sci Adv. 2021 Jul 28;7(31):eabh2169. doi: 10.1126/sciadv.abh2169. PMID: 34321199; PMCID: PMC8318366. (A single cell type gene expression atlas in human tissues. This study integrate single cell RNA sequencing results to classify the gene expression specificity in single cell type levels)

Sjöstedt E, Zhong W, Fagerberg L, Karlsson M, Mitsios N, Adori C, Oksvold P, Edfors F, Limiszewska A, Hikmet F, Huang J, Du Y, Lin L, Dong Z, Yang L, Liu X, Jiang H, Xu X, Wang J, Yang H, Bolund L, Mardinoglu A, Zhang C, von Feilitzen K, Lindskog C, Pontén F, Luo Y, Hökfelt T, Uhlén M, Mulder J. An atlas of the protein-coding genes in the human, pig, and mouse brain. Science. 2020 Mar 6;367(6482):eaay5947. doi: 10.1126/science.aay5947. PMID: 32139519. (This study reported the first systematic classification of gene expression specificity and distribution of all protein-coding genes in the mouse, pig and human brain.)

Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen LA, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P. Single-Cell Transcriptome Atlas of Murine Endothelial Cells. Cell. 2020 Feb 20;180(4):764-779.e20. doi: 10.1016/j.cell.2020.01.015. Epub 2020 Feb 13. PMID: 32059779. (This study reported the first investigation of EC heterogeneity in mouse tissues/organs using single cell transcriptome analysis)

Niu D, Wei HJ, Lin L, George H, Wang T, Lee IH, Zhao HY, Wang Y, Kan Y, Shrock E, Lesha E, Wang G, Luo Y, Qing Y, Jiao D, Zhao H, Zhou X, Wang S, Wei H, Güell M, Church GM, Yang L. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. Science. 2017 Sep 22;357(6357):1303-1307. doi: 10.1126/science.aan4187. Epub 2017 Aug 10. PMID: 28798043; PMCID: PMC5813284. (The first PERV inactivated pigs were generated by CRISPR and cloning.)

Full publications

Chen, L., Li, H., Teng, J., Wang, Z., Qu, X., Chen, Z., Cai, X., Zeng, H., Bai, Z., Li, J., Pan, X., Yan, L., Wang, F., Lin, L., Luo, Y., Sahana, G., Lund, M. S., Ballester, M., Crespo-Piazuelo, D. ... Yi, G. (2026). Construction of a Multitissue Cell Atlas Reveals Cell-Type-Specific Regulation of Molecular and Complex Phenotypes in Pigs. Advanced Science, 13(8), Article e04961. https://doi.org/10.1002/advs.202504961
Ortiz-Bueno, M., Zinghirino, F., Serra, P. P., Paschoudi, K., Montoliu, L., Atilla, E., Luo, Y., Cavazza, A., Lederer, C. W. & Benabdellah, K. (2026). From Bench to Bedside: Ethical and Clinical Best Practices for Genome Editing Applications. International Journal of Molecular Sciences , 27(3), Article 1484. https://doi.org/10.3390/ijms27031484
Ogurlu, B., van Furth, L. A., Zuo, Y., Hamelink, T. L., van Klinken, J. B., Pamplona, C. C., Bennedsgaard, S. S., van Leeuwen, L. L., Lantinga, V. A., Qi, H., Pool, M. B. F., Vendrell, I., Kessler, B. M., Fischer, R., Lin, L., Luo, Y., Jespersen, B., van Dijk, K. W., Bakker, B. M. ... Moers, C. (2026). Kidney Metabolism During Normothermic Machine Perfusion Differs Substantially From In Vivo Conditions. Transplantation, 110(2), e405-e415. https://doi.org/10.1097/TP.0000000000005586
Cavazza, A., Molina-Estévez, F. J., Reyes, Á. P., Ronco, V., Naseem, A., Malenšek, Š., Pečan, P., Santini, A., Heredia, P., Aguilar-González, A., Boulaiz, H., Ni, Q., Cortijo-Gutierrez, M., Pavlovic, K., Herrera, I., de la Cerda, B., Garcia-Tenorio, E. M., Richard, E., Granados-Principal, S. ... Benabdellah, K. (2025). Advanced delivery systems for gene editing: A comprehensive review from the GenE-HumDi COST Action Working Group. Molecular Therapy Nucleic Acids, 36(1), Article 102457. https://doi.org/10.1016/j.omtn.2025.102457
Han, B., Li, H., Zheng, W., Zhang, Q., Chen, A., Zhu, S., Shi, T., Wang, F., Zou, D., Song, Y., Ye, W., Du, A., Fu, Y., Jia, M., Bai, Z., Yuan, Z., Liu, W., Tuo, W., Hope, J. C. ... Sun, D. (2025). A multi-tissue single-cell expression atlas in cattle. Nature Genetics, 57(10), 2546-2561. https://doi.org/10.1038/s41588-025-02329-5
Yuan, H., Song, C., Xu, H., Sun, Y., Anthon, C., Bolund, L., Lin, L., Benabdellah, K., Lee, C., Hou, Y., Gorodkin, J. & Luo, Y. (2025). An Overview and Comparative Analysis of CRISPR-SpCas9 gRNA Activity Prediction Tools. The CRISPR Journal, 8(2), 89-104. https://doi.org/10.1089/crispr.2024.0058
Shi, M., Méar, L., Karlsson, M., Álvez, M. B., Digre, A., Schutten, R., Katona, B., Vuu, J., Lindström, E., Hikmet, F., Jin, H., Yuan, M., Li, X., Yang, H., Song, X., Sjöstedt, E., Edfors, F., Oksvold, P., von Feilitzen, K. ... Zhang, C. (2025). A resource for whole-body gene expression map of human tissues based on integration of single cell and bulk transcriptomics. Genome Biology, 26(1), 152. Article 152. https://doi.org/10.1186/s13059-025-03616-4
Sun, Y., Qu, K., Corsi, G. I., Anthon, C., Pan, X., Xiang, X., Jensen, L. J., Lin, L., Luo, Y. & Gorodkin, J. (2025). Deep learning models simultaneously trained on multiple datasets improve base-editing activity prediction. Nature Communications, 16(1), Article 9821. https://doi.org/10.1038/s41467-025-65200-5
Liang, X., Arrey, G., Qin, Y., Álvarez-González, L., Hariprakash, J. M., Ma, J., Holt, S., Han, P., Luo, Y., Li, H., Ruiz-Herrera, A., Pilegaard, H. & Regenberg, B. (2025). EccDNA atlas in male mice reveals features protecting genes against transcription-induced eccDNA formation. Nature Communications, 16(1), 1872. Article 1872. https://doi.org/10.1038/s41467-025-57042-y
Li, C., Lv, W., He, Z., Pan, X., Zeng, Y., Mulder, J., Sjöstedt, E., Huang, Z., Liu, W., Xu, L., Eisenberg, E., Lin, L., Hansen, J. B., Huang, J. & Luo, Y. (2025). Landscape of A-I RNA editing in mouse, pig, macaque, and human brains. Nucleic Acids Research, 53(11), Article gkaf534. https://doi.org/10.1093/nar/gkaf534
Zeng, Y., Lv, W., Tao, H., Li, C., Jiang, S., Liang, Y., Chen, C., Yu, T., Li, Y., Wu, S., Cui, X., Liang, N., Wang, P., Xu, H., Dong, J., Teng, H., Chen, K., Mu, K., Fan, T. ... Han, P. (2025). Mapping the chromothripsis landscape in urothelial carcinoma unravels great intratumoral and intertumoral heterogeneity. iScience, 28(1), 111510. Article 111510. https://doi.org/10.1016/j.isci.2024.111510
Madan, E., Palma, A. M., Vudatha, V., Kumar, A., Bhoopathi, P., Wilhelm, J., Bernas, T., Martin, P. C., Bilolikar, G., Gogna, A., Peixoto, M. L., Dreier, I., Araujo, T. F., Garre, E., Gustafsson, A., Dorayappan, K. D. P., Mamidi, N., Sun, Z., Yekelchyk, M. ... Gogna, R. (2025). Ovarian tumor cells gain competitive advantage by actively reducing the cellular fitness of microenvironment cells. Nature Biotechnology, 43(11), 1833-1847. Article e13714. https://doi.org/10.1038/s41587-024-02453-3
Han, P., Zhang, C., Ge, F., Liu, C., Fu, J., Lv, W., Jin, X., Zhang, J., Zhao, L., Luo, Y., Su, X. & Lan, X. (2025). Plasma cell-free extrachromosomal circular DNA is a molecular hallmark of preeclampsia. Computational and Structural Biotechnology Journal, 27, 2602-2613. https://doi.org/10.1016/j.csbj.2025.06.020
Luo, Y. & COST Action CA21113 Members (2025). Proceedings of the second annual meeting of GenE-HumDi (COST Action 21113). Frontiers in Genome Editing, 7, Article 1667329. https://doi.org/10.3389/fgeed.2025.1667329
Yuan, H., Song, Z., Sun, X.-Q., Song, C., Guo, L., Zhang, Q., Luo, Y., Yuan, C., Gao, J. & Jia, X. (2025). Profiling the cell diversity and tissue structure of aqueous humor circulatory system in human eyes using spatial single-cell RNA sequencing. Genes & diseases, 12(1), 101304. Article 101304. https://doi.org/10.1016/j.gendis.2024.101304
Koning, M., Dumas, S. J., Meta, E., Lievers, E., de Graaf, A. M. A., Borri, M., Nai Chung Tong, L. J., Liang, X., Liu, P., Chen, F., Lin, L., Luo, Y., Carmeliet, P., van den Berg, C. W. & Rabelink, T. J. (2025). Single cell transcriptomics of human kidney organoid endothelium reveals vessel growth processes and arterial maturation upon transplantation. npj Regenerative Medicine, 10(1), Article 32. https://doi.org/10.1038/s41536-025-00418-x
Lv, W., Zeng, Y., Li, C., Liang, Y., Tao, H., Zhu, Y., Sui, X., Li, Y., Jiang, S., Gao, Q., Rodriguez-Fos, E., Prasad, G., Wang, Y., Zhou, R., Xu, Z., Pan, X., Chen, L., Xiang, X., Teng, H. ... Han, P. (2025). Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment. Cancer Discovery, 15(6), 1225-1246. https://doi.org/10.1158/2159-8290.CD-24-1532
Gultom, M., Lin, L., Brandt, C. B., Milusev, A., Despont, A., Shaw, J., Döring, Y., Luo, Y. & Rieben, R. (2025). Sustained Vascular Inflammatory Effects of SARS-CoV-2 Spike Protein on Human Endothelial Cells. Inflammation, 48(4), 2531–2547. https://doi.org/10.1007/s10753-024-02208-x
FarmGTEx Consortium (2025). The Farm Animal Genotype-Tissue Expression (FarmGTEx) Project. Nature Genetics, 57(4), 786-796. Article 604. https://doi.org/10.1038/s41588-025-02121-5
Luo, Y., Ceasar, S. A. & Benabdellah, K. (2025). Trajectory of genome editing technology. BMC Biology, 23(1), 351. Article 351. https://doi.org/10.1186/s12915-025-02450-1
Gao, P., Li, S., Dong, Z., Luo, Y., Zhang, X., Han, L., Peng, S., Shen, J., Xu, F. & Deng, Z. (2024). A plasma-derived exosomal microRNA signature by small RNA sequencing for early detection of postmenopausal osteoporosis. Clinical and Translational Medicine, 14(4), Article e1637. https://doi.org/10.1002/ctm2.1637
Lv, W., Pan, X., Han, P., Wu, S., Zeng, Y., Wang, Q., Guo, L., Xu, M., Qi, Y., Deng, L., Xu, Z., Li, C., Yu, T., Cui, X., Teng, H., Xiang, C., Tan, H., Li, Y., Liang, N. ... Luo, Y. (2024). Extrachromosomal circular DNA orchestrates genome heterogeneity in urothelial bladder carcinoma. Theranostics, 14(13), 5102-5122. https://doi.org/10.7150/thno.99563
Huang, F., Lyu, B., Xie, F., Li, F., Xing, Y., Han, Z., Lai, J., Ma, J., Zou, Y., Zeng, H., Xu, Z., Gao, P., Luo, Y., Bolund, L., Tong, G. & Fengping, X. (2024). From gut to liver: unveiling the differences of intestinal microbiota in NAFL and NASH patients. Frontiers in Microbiology, 15, Article 1366744. https://doi.org/10.3389/fmicb.2024.1366744
Recknagel, H., Močivnik, L., Zakšek, V., Luo, Y., Kostanjšek, R. & Trontelj, P. (2024). Generation of genome-wide SNP markers from minimally invasive sampling in endangered animals and applications in species ecology and conservation. Molecular Ecology Resources, 24(7), Article e13995. https://doi.org/10.1111/1755-0998.13995
Gál, Z., Boukoura, S., Oxe, K. C., Badawi, S., Nieto, B., Korsholm, L. M., Geisler, S. B., Dulina, E., Rasmussen, A. V., Dahl, C., Lv, W., Xu, H., Pan, X., Arampatzis, S., Stratou, D.-E., Galanos, P., Lin, L., Guldberg, P., Bartek, J. ... Larsen, D. H. (2024). Hyper-recombination in ribosomal DNA is driven by long-range resection-independent RAD51 accumulation. Nature Communications, 15(1), Article 7797. https://doi.org/10.1038/s41467-024-52189-6
Lebas, M., Chinigò, G., Courmont, E., Bettaieb, L., Machmouchi, A., Goveia, J., Beatovic, A., Van Kerckhove, J., Robil, C., Angulo, F. S., Vedelago, M., Errerd, A., Treps, L., Gao, V., Delgado De la Herrán, H. C., Mayeuf-Louchart, A., L'homme, L., Chamlali, M., Dejos, C. ... Cantelmo, A. R. (2024). Integrated single-cell RNA-seq analysis reveals mitochondrial calcium signaling as a modulator of endothelial-to-mesenchymal transition. Science Advances, 10(32), eadp6182. Article adp6182. https://doi.org/10.1126/sciadv.adp6182
Zhou, M., Lv, W., Han, P., Sun, K., Hao, Z., Gao, L., Xu, Y., Xu, Z., Shao, S., Ma, S., Guo, Q., Zhang, H., Liu, K., Yang, F., Yuan, Z., Wu, G., Yu, C., Luo, Y., Yao, Z. & Zhao, J. (2024). Plasma extrachromosomal circular DNA as a potential diagnostic biomarker for nodular thyroid disease. Clinical and Translational Medicine, 14(6), e1740. https://doi.org/10.1002/ctm2.1740
Cham, L. B., Rosas-Umbert, M., Lin, L., Tolstrup, M. & Søgaard, O. S. (2024). Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 58(4), 322-335. https://doi.org/10.33594/000000715
Skorda, A., Lauridsen, A. R., Wu, C., Huang, J., Mrackova, M., Winther, N. I., Jank, V., Sztupinszki, Z., Strauss, R., Bilgin, M., Maeda, K., Liu, B., Luo, Y., Jäättelä, M. & Kallunki, T. (2023). Activation of invasion by oncogenic reprogramming of cholesterol metabolism via increased NPC1 expression and macropinocytosis. Oncogene, 42(33), 2495-2506. https://doi.org/10.1038/s41388-023-02771-x
Faienza, F., Polverino, F., Rajendraprasad, G., Milletti, G., Hu, Z., Colella, B., Gargano, D., Strappazzon, F., Rizza, S., Vistesen, M. V., Luo, Y., Antonioli, M., Cianfanelli, V., Ferraina, C., Fimia, G. M., Filomeni, G., De Zio, D., Dengjel, J., Barisic, M. ... Cecconi, F. (2023). AMBRA1 phosphorylation by CDK1 and PLK1 regulates mitotic spindle orientation. Cellular and Molecular Life Sciences, 80(9), Article 251. https://doi.org/10.1007/s00018-023-04878-6
Liu, B., Chen, R., Zhang, Y., Huang, J., Luo, Y., Rosthøj, S., Zhao, C. & Jäättelä, M. (2023). Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ efflux. Cell Reports, 42(2), Article 112137. https://doi.org/10.1016/j.celrep.2023.112137