1. A CRISPR-C technology developed by AU-iCRISPR network.
Internal collaborative groups: Yonglun Luo, Eigil Kjeldsen, Uffe Birk Jensen
External collaborative groups: Huanming Yang (BGI), George M. Church (Harvard), Birgitte Regenberg (KU)
Publication:
Henrik Devitt Møller, Lin Lin, Xi Xiang, Trine Skov Petersen, Jinrong Huang, Luhan Yang, Eigil Kjeldsen, Uffe Birk Jensen, Xiuqing Zhang, Xin Liu, Xun Xu, Jian Wang, Huanming Yang, George M. Church, Lars Bolund, Birgitte Regenberg, Yonglun Luo #. (2018) CRISPR-C: Circularization of genes and chromosome by CRISPR in human cells. Nucleic Acid Research. https://doi.org/10.1093/nar/gky767. [Epub ahead of print] (*)
Related news releases from the study:
2. CRISPR aids discovery of Nrf2 function in STING regulation
Internal collaborative groups: Martin R. Jakobsen, Thomas B. Poulsen, Christian K. Holm, Yonglun Luo, Mette Nyegaard
Publication:
David Olagnier, Aske M. Brandtoft, Camilla Gunderstofte, Nikolaj L. Villadsen, Christian Krapp, Anne L. Thielke, Anders Laustsen, Suraj Peri, Anne Louise Hansen, Lene Bonefeld, Jacob Thyrsted, Victor Bruun, Marie B. Iversen, Lin Lin, Virginia M. Artegoitia, Chenhe Su, Long Yang, Rongtuan Lin, Siddharth Balachandran,Yonglun Luo, Mette Nyegaard, Bernadette Marrero, Raphaela Goldbach-Mansky, Mona Motwani, Dylan D. Ryan, Katherine A. Fitzgerald, Luke A. O'Neill, Anne K. Hollensen, Christian K. Damgaard, Frank v. de Paoli, Hanne C. Bertram, Martin R. Jakobsen, Thomas B. Poulsen, Christian K. Holm. (2018) Nrf2 is a negative regulator of STING during metabolic reprogramming. Nature Communications. 9, Article number: 3506 (2018)
3. Book chapters from AU-iCRISPR network
Methods in Molecular Biology: CRISPR Gene Editing - Methods and Protocols (Edited by Yonglun Luo)
TABLE OF CONTENT (TOC):
Section 1. Methods for CRISPR-gRNA design and quantification of activity
1. Maria Pallarès Masmitjà, Nastassia Johanna Knödlseder, and Marc Güell. CRISPR gRNA design
2. Lin Lin and Yonglun Luo. Tracking CRISPR’s Footprints (AU)
3. Eva Karina Brinkman and Bas van Steensel. Rapid quantitative evaluation of CRISPR genome editing by TIDE and TIDER
4. Saskia König, Yang Zhang, Hans Heugh Wandall, Claudio Mussolino, and Eric Paul Bennett. Fast and quantitative identification of ex vivo precise genome targeting induced indel events by IDAA
5. Lin Lin and Yonglun Luo. Functional evaluation of CRISPR activity by the dual-fluorescent surrogate system: C-Check (AU)
Section 2. Methods for CRISPR delivery
6. Suleixin Yang, Qinjie Wu, Yuquan Wei, and Changyang Gong. CRISPR-Cas9 delivery by artificial virus (RRPHC)
7. Laura Barrett Ryø, Emil Aagaard Thomsen, and Jacob Giehm Mikkelsen. Production and validation of lentiviral vectors for CRISPR/Cas9 delivery (AU)
8.Julia Fakhiri, Manuela Nickl, and Dirk Grimm. Rapid and simple screening of CRISPR g(uide)RNAs in cultured cells using Adeno-associated viral (AAV) vectors
9. Anders Laustsen and Rasmus O. Bak. Electroporation-based CRISPR/Cas9 gene editing using Cas9 protein and chemically modified sgRNAs (AU)
Section 3. CRISPR gene editing in human iPSCs
10. Saniye Yumlu, Sanum Bashir, Jürgen Stumm, Ralf Kühn. Efficient gene editing of human induced pluripotent stem cells using CRISPR/Cas9
11: Bruntraeger, M., Byrne, M., Long, K., Bassett, A.R. Editing the genome of human induced pluripotent stem cells using CRISPR/Cas9 ribonucleoprotein complexes
12. Kirsten E. Snijders, James D. Cooper, Ludovic Vallier, and Alessandro Bertero. Conditional gene knockout in human cells with inducible CRISPR/Cash (AU)
Section 4. CRISPR gene editing in other cell types
13. Daria Sergeeva, Jose Manuel Camacho-Zaragoza, Jae Seong Lee, and Helene Faustrup Kildegaard. CRISPR/Cas9 as a genome editing tool for targeted gene integration in CHO cells
14. Signe Neldeborg, Lin Lin, Magnus Stougaard, Yonglun Luo. Rapid and efficient gene deletion by CRISPR-Cas9 (AU)
15. Lisbeth Ahm Hansen and Ernst-Martin Füchtbauer. Genome editing in mice (AU)
16. Xi Xiang, Conghui Li, Xi Chen, Hongwei Dou, Yong Li, Xiuqing Zhang, Yonglun Luo. CRISPR/Cas9 Mediated Gene Tagging: A Step-by-Step Protocol (AU)
17. Petra Vochozkova, Kilian Simmet, Eva-Maria Jemiller, Annegret Wünsch, Nikolai Klymiuk. Gene Editing in primary cells of cattle and pig
Section 5. CRISPR gene therapy and screening
18. Kristian Alsbjerg Skipper and Jacob Giehm Mikkelsen. Toward in vivo gene therapy using CRISPR (AU)
19. Andreas Holmgaard, Sidsel Alsing, Anne Louise Askou and Thomas J. Corydon. CRISPR gene therapy of the eye: Targeted knockout of Vegfa in mouse retina by lentiviral delivery (AU)
20. Francesco Niola, Frederik Dagnæs-Hansen and Morten Frödin. In vivo editing of the adult mouse liver using CRISPR/Cas9 and hydrodynamic tail vein injection
21: Emil Aagaard Thomsen and Jacob Giehm Mikkelsen. CRISPR-based lentiviral knockout libraries for functional genomic screening and identification of phenotype-related genes (AU)